Neurite length measurement can be done in three ways with ImageJ:

| Method | Best for | Accuracy | Time | |--------|----------|----------|------| | | Low cell density, validation | ★★★★★ | Very slow | | Semi-automated (NeuronJ plugin) | Moderate density, branching | ★★★★☆ | Moderate | | Fully automated (Macro + Skeletonize) | High throughput, simple morphologies | ★★★☆☆ | Fast |