For the vast majority of life sciences and biotechnology contexts, . Would you like a protocol for cellulase activity assay or for reviving a frozen culture of this strain?
| Parameter | Recommended Range | |-----------|------------------| | | 1–2% Avicel (microcrystalline cellulose), Solka Floc, or lactose (2–4%) | | Carbon source (growth) | 0.5–1% glucose (but will repress cellulase production) | | Nitrogen source | 0.2–0.5% peptone + (NH₄)₂SO₄ | | pH | 4.5–5.0 (optimal for growth & secretion) | | Temperature | 28–30°C | | Agitation | 150–250 rpm (aeration important) | | Time to peak enzyme | 5–7 days in shake flasks | Tip: To achieve high cellulase titers, start with a small amount of glucose for initial growth, then shift to cellulose or lactose. Avoid using >0.5% glucose during enzyme production phase. 5. Comparison with Other T. reesei Strains | Strain | Cellulase Yield (Relative) | Catabolite Repression | β-glucosidase | Typical Use | |--------|----------------------------|------------------------|---------------|--------------| | QM6a (wild) | 1x (baseline) | Strong | Low | Original isolate | | Mogi 131 | ~5–10x | Partial | Low | High-cellulase research | | Rut-C30 | ~15–20x | Very weak | Very low | Industrial biofuel strain | | CL847 | ~12–15x | Moderate | Moderate | Lab & biorefinery models |